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KMID : 1140120110160010041
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Cancer Prevention Research
2011 Volume.16 No. 1 p.41 ~ p.50
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Glutamine Deprivation Induces Growth Inhibition in Human Prostate Carcinoma LnCap Cells
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Shin Dong-Yeok
Choi Yung-Hyun
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Abstract
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While the amino acid L-glutamine, a most abundant amino acid in the blood, is known to play a role in the survival and death of several cell types, the underlying molecular mechanisms are still poorly defined. The purpose of this study is to determine whether glutamine deprivation induces growth inhibition and apoptosis in human prostate carcinoma LnCap cells. Our data indicated that glutamine deprivation triggered a progressive reduction in the cell viability through arresting the cell cycle in the S phase. This process involved the increase of sub-G1 phase DNA contents, however chromatin condensation as well as DNA fragmentation were not detected in cells cultured in the absence of glutamine indicating that glutamine deprivation did not induce apoptosis in LnCap cells. Supporting the data, the several important apoptosis-related gene products such as death receptor-related genes, IAP and Bcl-2 family members, and caspases, were not changed or activated. Moreover, the level poly-(ADP-ribose) polymerase, β-catenin and phospholipase C-γ1 expression, activated caspase-3 target proteins, remained unchanged in response to glutamine deprivation. This study provides biochemical evidence that glutamine deprivation suppresses cell viability without induction of apoptosis in human prostate carcinoma LnCap cells.
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KEYWORD
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Glutamine deprivation, LnCap, Cell cycle, Apoptosis
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